That feature is only available with FileLocator Pro but you might be able to achieve what you want by either specifying the specific folders, e.g.
C:\Folders\Sub1;C:\Folders\Sub4
Or by specifying a file name criteria, e.g.
*.txt;*.doc
More info: How does FileLocator Pro exclusions work?
With FileLocator Pro you can exclude a specific folder by prefixing the name with an exclamation mark, e.g.
C:\Folders;!C:\Folders\Sub3
Or you can exclude a group of subfolders based on a term by prefixing the term with a minus sign, e.g. to exclude any sub folder with the word Archive:
C:\Folders;-Archive
Or conversely to ONLY search subfolders which contain a specific term in the name prefix the term with a plus sign, e.g. to only search subfolders with the word Work in the path:
C:\Folders;+Work